Challenges
| Name | Character | Starting items | Disabled rooms | Curse filters | Additional notes | End goal | Unlock condition | Reward |
|---|---|---|---|---|---|---|---|---|
| 1. [Eclipsed] When life gives you Potatoes! | Isaac | Potato Peeler
| - | All items turn into Potato during this challenge. | Mom's Heart | - | Cyber Cutlet | |
| 2. [Eclipsed] Curse of The Magician | Isaac | Spoon Bender
Telekinesis | - | All enemies get homing tears during this challenge. | Mom | - | Mew-Gen | |
| 3. [Eclipsed] Lobotomy | Tainted Eden | Missing No.
D100 Error | Enemies, rocks and background rerolled when entering room. | Mom | - |  Delirious Bum, Delirious Pickups | ||
| 4. [Eclipsed] Beatmaker | The Lost No HP. | Heart Transplant | - | - | Heart Transplant always shoot 10 tears. | Mom's Heart | - | Gilded Fork |
| 5. [Eclipsed] Mongo Family | Isaac | Mongo Cells
Adoption Papers | - | +15¢. Replaces all slot machines and beggars with  Mongo Beggar during this challenge. | Mom's Heart | - | Golden Egg | |
| 6. [Eclipsed] Rise of Khepri | Isaac | Skatole
Bursting Sack Friend Finder Khepri Key Piece 1 Key Piece 2 | - | Friend Finder gets timed charge with 10 seconds. | Mega Satan | - | Agony Box | |
| 7. [Eclipsed] Ritualist | Tainted Judas | Ceremonial Robes
The Battery Nirly's Codex, Dark Arts | - | Dark Arts activates Ritual Dagger. | Mom's Heart | - | Wild Curse | |
| 8. [Eclipsed] Eco Portals |  Eco | Cosmic Spot
0 - The Fool Key Piece 1 Key Piece 2 Echo Room | - | - | Doors replaced by portals during this challenge.
When a room is cleared, 1 to 3 portals are created, leading to the next unexplored rooms and 1 portal leading to the starting room. Portals can be hidden by holding the map button (Tab). Echo Room has 3 max.charge and saves room where it was used, so it can be revisited. Portals to saved rooms appear in starting room. | Mega Satan | - | Cosmic Spot |
| 9. [Eclipsed] Heartcarver |  Magdalene | Aurora
Holy Ravioli Secret Love Letter | - | - | Mom's Heart | - |  Fool of Hearts | |
| 10. [Eclipsed] Call of Lutos | Isaac | Lutos
Elder Sign | - | - | Mom | - | Lilac Clef | |
| 11. [Eclipsed] Pilgrimage | Lazarus | The Battery
Gospel Expurgation Penance | - | - | Satan | - | Soul in a Jar | |
| 12. [Eclipsed] Die Hard | Lazarus | False Certificate
| - | Infinite Basement! | Satan | - | False Certificate |